Journal: Bioactive Materials

Article Title: A large puncture closer of aortic wall by multi-memory actions with thrombo-hemodynamic control

doi: 10.1016/j.bioactmat.2025.12.042

Figure Lengend Snippet: 18 Fr puncture of hemostasis in porcine aorta using VWP by validating the memory programming effect of each part. a, As a challenging model for application of large-diameter catheters, i) an 18 Fr (6 mm) puncture is created into the porcine thoracic aorta (diameter: 10 mm) so that the size-matched VWP is deployed, followed by measuring proximal and distal blood pressure. ii) The experimental groups are designed first to exam the memory programming effect of collaboration between Ring squeezing with Body expansion on self-locking (SL) to enable efficient hemostasis. Next, the effect of Wing shape recovery from curve to flat is examined on hemodynamic control (HC) in cooperation with the actions of Body and Ring to handle hemostasis. No recovery of Wing shape is expected to induce excessive thrombosis. iii) Four experimental groups are established using a total of 12 pigs (N = 12) with immediate sacrifice following deployment (N = 3 each). Group 1 [SL(−) HC(−)] represents no memory programming. Group 2 [SL(+) w/flat Wing] has the effects of Body and Ring actions except the hemostatic sealing by keeping the flat Wing. Group 3 [SL(+) HC(+)] possesses the complete memory effects of the three parts. Group 4 [SL(+) w/bump Wing] is expected to have excessive thrombosis because of no shape recovery from the curved Wing while maintaining the memory actions of Body and Wing. b, Each group is visually explained in the illustrations. c , In VWP actions, (left) the bleeding condition preserves the normal sinusoidal waveform of high proximal pressure (green) in contrast to the disturbed waveform of low distal pressure (red). (middle) Hemostatic closure results in similar high sinusoidal waveform at both pressure sites. (right) Excessive thrombosis does not disturb the waveform, but the distal pressure level becomes lower than the proximal one. d, When reperfusion starts by removing the clamp post-deployment (blue), only Group 3 [SL(+) HC(+)] reaches the hemostatic closure, as evidenced by flow stabilization (red) with a 5 s plateau at both pressure sites. Group 4 [SL(+) w/bump Wing] exhibits the pattern of over-thrombosis. e, H&E images show bleeding in Group 1 as an indication of incomplete closure in contrast to moderate, minimal, and dense thrombotic features observed in Group 2, 3, and 4 respectively as further supported by the signals of activated platelets (green, CD41-positive) and fibrinogen (red) [Scale bars = 0.5 mm (4 mm in box)]. f, Compared to Group 1 [SL(−) HC(−)] and 2 [SL(+) w/flat Wing], Group 3 [SL(+) HC(+)] shows the fastest i) hemostasis and ii) arterial pressure equilibration, indicating the most efficient hemostatic response. g, These outcomes in Group 3 include i) the smallest difference between the proximal and distal pressures with ii) the smallest thrombus area in contrast the largest area of Group 4 [SL(+) w/bump Wing] as an indication of excessive thrombosis. h , The marker gene expression of thrombotic feature (vWF, PF-4, and P-sel) significantly increases from Group 2 to Group 3 and further to Group 4 except the comparison of vWF expression between Group 2 and 3 (ns: no significance). Data are shown as mean ± SD, N = 3 biologically independent animals per group. Significance was determined using one-way ANOVA with Tukey's test between groups.

Article Snippet: Primary antibodies against CD41 (1:100, 24552-1-AP, proteintech), fibrinogen (1:100, ab232793, Abcam), CD31 (1:100, sc-376764, Santa Cruz Biotechnology), CD68 (1:100, ab125212, Abcam), and ARG-1 (1:200, LS-C447907, LSBio) were applied overnight at 4°C.

Techniques: Control, Marker, Gene Expression, Comparison, Expressing

Journal: bioRxiv

Article Title: Activating FGFR1 restores Integrin-β1–mediated fibronectin sensing in satellite cells of aged mice

doi: 10.64898/2026.02.18.706475

Figure Lengend Snippet: (A) Experimental scheme for treatment of young caFGFR1– mice with tamoxifen and doxycycline chow, followed by myofiber isolation and culture prior to fixation. (B) Representative images of myofibers cultured with or without FGF-2, in the presence of AIIB2 or TS2/16, where SCs were identified by Syndecan-4 immunoreactivity. Scale bars, 100 μm. (C) Number of Syndecan-4 + cells on myofibers cultured with or without FGF-2, in the presence of AIIB2 or TS2/16. n > 10 myofibers analyzed with samples from 3 mice, and statistics performed based on 3 independent mice; data are presented as mean ± SD; **P < 0.01 and ***P < 0.001 in a two-way ANOVA test. (D) Representative images of SCs on myofibers cultured with or without FGF-2, in the presence of AIIB2 or TS2/16, identified by Syndecan-4 immunoreactivity and assessed for phospho-histone H3 (pHH3) and PARD3 immunoreactivity. Scale bars, 10 μm. (E and F) Percentage of pHH3 + immunoreactive cells (E) and percentage of asymmetric division (F) on myofibers cultured with or without FGF-2, in the presence of AIIB2 or TS2/16. n > 200 Syndecan-4 + cells analyzed with samples from 3 mice, and statistics performed based on 3 independent mice; data are presented as mean ± SD; *P < 0.05 and ***P < 0.001 in a two-way ANOVA test.

Article Snippet: When indicated, myofibers were either fixed in 4% paraformaldehyde for 10 min immediately after isolation or cultured in suspension at 37 °C and 5% CO2 for 36 h prior to fixation or 24 h prior to encapsulation, with supplementation of 0.5 nM fibroblast growth factor 2, 1 μg/mL AIIB2 (Developmental Studies Hybridoma Bank), or 1 μg/mL TS2/16 (eBioscience) as specified.

Techniques: Isolation, Cell Culture

Journal: bioRxiv

Article Title: Activating FGFR1 restores Integrin-β1–mediated fibronectin sensing in satellite cells of aged mice

doi: 10.64898/2026.02.18.706475

Figure Lengend Snippet: (A) Experimental scheme showing treatment of aged caFGFR1– and caFGFR1+ mice and the timeline for myofiber harvest and culture. (B) Representative images of myofibers from aged caFGFR1–and caFGFR1+ mice cultured in the presence of AIIB2 antibody or TS2/16 antibody, where SCs were identified by Syndecan-4 immunoreactivity. Scale bars, 100 μm. (C) Syndecan-4 + cells on myofibers quantified from aged caFGFR1– and caFGFR1+ mice cultured in the presence of AIIB2 or TS2/16. n > 10 myofibers analyzed with samples from 3 mice, and statistics performed based on 3 independent mice; data are presented as mean ± SD; *P < 0.05 and ***P < 0.001 in a two-way ANOVA test. (D) Representative images of SCs on myofibers from aged caFGFR1– and caFGFR1+ mice cultured in the presence of AIIB2 antibody or TS2/16 antibody, where SCs were identified by Syndecan-4 immunoreactivity and assayed for pHH3 immunoreactivity and PARD3 immunoreactivity. Scale bars, 10 μm. (E and F) The percentage of pHH3 + cells (E) and percentage of asymmetric division (F) on myofibers from aged caFGFR1– and caFGFR1+ mice cultured in the presence of AIIB2 or TS2/16. n > 200 Syndecan-4 + cells analyzed with samples from 3 mice, and statistics performed based on 3 independent mice; data are presented as mean ± SD; *P < 0.05, **P < 0.01, and ***P < 0.001 in a two-way ANOVA test.

Article Snippet: When indicated, myofibers were either fixed in 4% paraformaldehyde for 10 min immediately after isolation or cultured in suspension at 37 °C and 5% CO2 for 36 h prior to fixation or 24 h prior to encapsulation, with supplementation of 0.5 nM fibroblast growth factor 2, 1 μg/mL AIIB2 (Developmental Studies Hybridoma Bank), or 1 μg/mL TS2/16 (eBioscience) as specified.

Techniques: Cell Culture